Original Research Contributions in Genetic Biongineering And Natural Genome Editing
Museum program design supports parent-child engineering talk during tinkering and reminiscing
This study focused on fiddling, funny form of open-solving that is being adopted widely in science, technology, engineering and math (STEM) education as a way to encourage the involvement of children in the practice of engineering discipline.
However, the design of exhibitions and programs and the nature of the interaction of children with adults can determine whether and to what extent tinkering raises participation in engineering practices such as testing and redesign. Museum researchers and practitioners work together using design-based research to develop and test programs that could tamper with the best study engineering support.
Two of the programs is determined what family should undertake engineering projects and provide exhibition space for testing and design iterations (ie, function-focused programs), and the two programs do not. A total of 61 families with children 6 to 8 years (Mage = 7:07 years; 25 women) were observed for any of the programs and when recalled immediately after the play. parent-child interaction patterns associated with understanding and remembering the combined hands-parent-child events and joint involvement talk and talk the engineering design process is measured.
All four similar programs in terms of joint parent-child involvement. Compared with families who do not participate in function-focused program, families who do not speak more about the engineering design process for tinkering and when recalled. Parent-child talk engineered for tinkering mediated the relationship between design and engineering program speech when recalled. Implications for research on teaching practices and a children’s museum are discussed.
Museum program design supports parent-child engineering talk during tinkering and reminiscing
3D rapid prototyping Electrochemistry Organic Transistor by photocurable composite resin
Rapid Prototyping (RP) promises to induce a revolutionary impact on how objects can be produced and used in the manufacturing industry as well as in everyday life. Over time the standard technique as 3D Stereolithography (SL) has become a fundamental technology for RP and Additive Manufacturing (AM), because it allows the fabrication of 3D objects from photocurable resin cost-effective.
Efforts to get a device that is more complex than just simulacre aesthetic, has spent with an uncertain outcome. The multidisciplinary nature of the manufacturing techniques such as furtherly blocking the route to the fabrication of complex devices. A good knowledge of the fundamentals of materials science and engineering required to handle the SL technology, characterization and testing aspects.
Within this framework, the purpose of our research to reveal new approaches to get RP complex devices, namely Organic Electro-Chemical Transistor (OECTs), the SL technique exploits conductive resin composites based on poly (3,4-ethylenedioxythiophene): polystyrene sulfonate (PEDOT: PSS) and a photo-curable poly (ethylene glycol) diacrylate (PEGDA). A comprehensive study were presented, ranging from the optimization of composite resins and characterization of electrochemical properties, up to 3D printing OECTs and testing. show relevant in biosensing for dopamine (DA) detection using 3D OECTs too.
Description: This product includes one 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins.
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Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included.
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.
For reported and discussed over the last two decades, there has been research going on to develop advanced energy technologies involving minimum environmental pollution, to replace conventional fossil energy system. Solid oxide fuel cells (SOFCs) is one of the most promising, environmentally friendly and efficient means for the generation of electricity to meet the energy needs of the future.
